Corrigendum: Validation and Development of COI Metabarcoding Primers for Freshwater Macroinvertebrate Bioassessment

Corrigendum: Validation and Development of COI Metabarcoding Primers for Freshwater Macroinvertebrate Bioassessment

In the original article, the reference for Bista et al. (2017) was incorrectly written as “Carvalho, G. R., Walsh, K., Seymour, M., Hajibabaei, M., Lallias, D., Christmas, M., et al. (2017). Annual time-series analysis of aqueous eDNA reveals ecologically relevant dynamics of lake ecosystem biodiversity. Nat. Commun. 8, 1–11. doi: 10.1038/ncomms14087.” It should be “Bista, I., Carvalho, G. R., ...

Validation and Development of COI Metabarcoding Primers for Freshwater Macroinvertebrate Bioassessment

Short COI markers for freshwater macroinvertebrate metabarcoding

Species diversity of metazoan bulk samples can be rapidly assessed using Cytochrome c oxidase I (COI) metabarcoding. However, in cases where only degraded DNA is available, e.g. from poorly conserved museum specimens, eDNA filtered from water or gut content analyses, universal primer sets that amplify only a short COI fragment are advantageous. Using PrimerMiner, we optimised two universal prim...

Evaluating subsampling approaches and macroinvertebrate taxonomic resolution for wetland bioassessment

Methods for wetland bioassessment using macroinvertebrates are not well developed. Two of the most controversial issues in stream bioassessment, subsampling and taxonomic resolution, have yet to be quantitatively addressed for wetlands. Using a multivariate approach, we evaluated the efficacy of family-, genus-, and species-level assemblage data in reflecting the environment and distinguishing ...

Next-generation freshwater bioassessment: eDNA metabarcoding with a conserved metazoan primer reveals species-rich and reservoir-specific communities

Freshwater habitats are of high conservation value and provide a wide range of ecosystem services. Effective management requires regular monitoring. However, conventional methods based on direct observation or specimen collection are so invasive, expensive and labour-intensive that frequent monitoring is uncommon. Here, we test whether the evaluation of environmental DNA (eDNA) from water based...